PH0975 | 戴维森氏固定液 Davidson's Fixative/Hartmann's fixative

Hartman固定剂，也称为Davidson固定剂，含有醋酸、酒精和福尔马林。它被广泛用于保存眼、骨髓、乳房、睾丸和其他组织以便组织学检查。

用作根治性切除标本中淋巴结可视化的过夜固定剂。通过将淋巴结转变为白色，有助于乳房和结肠标本检查。Moore和Barr使用了一种Hartman固定剂来固定皮肤活检标本，用于研究细核细节。使用常规和改良的Hartman固定剂将大鼠睾丸固定24小时以用于免疫组织化学和形态学比较，而将用于其他分析的眼睛和睾丸标本固定48小时。

Description

Davidson's fixative (sometimes called Hartmann's fixative) is a rapid fixative. It is particularly useful for preparing tumors, bone marrow specimens, gynecologic material, fatty breast, and medical biopsies. It may be used for testicularbiopsies and for overnight fixation of fat containing lymph nodes. In addition, Davidson Solution is the preferred fixative for whole eyes, which provides optimal tissue preservation while avoiding retinal detachment.

Storage: RT

Store in closed container (plastic or glass) to prevent evaporation.

Protocol

1. Use about 100 ml for bigger objects or several small specimens. Place specimen in the solution immediately after extraction. (A gauze pad should be used to keep floating objects submerged if necessary or inconsistent fixation will occur.)

2. Remain specimen in the solution for 1 -2 days. Important note: Fixation of small specimens is rapid. Exposure to the fixative should be limited to 24 hours (tissue may be transferred to neutral buffered formalin or 70% alcohol for storage).

3. The specimen then is taken out of the solution and placed either in formalin or 70% ethanol. Optional : To make very solid specimen (such as eyes) easier to cut, incubate in 70% ethanol for a day, transfer to 90% ethanol for a day and finally to 100% ethanol for a day.

Safety precautions:

1. Wear safety glasses, a lab coat, and gloves.

2. Prepare in area with adequate ventilation and away from heat or open flame.