PH1379 | Von Ebener's液 脱钙液

产品简介：

在组织切片过程中一些组织内含有骨质或钙化灶时，含钙的组织不宜直接用石蜡包埋切片，这是因为钙和石蜡之间的密度不同，较难切出完整的切片。对含钙组织最好固定之后，再进行脱钙或二者同时进行，然后进行下游操作如脱水、透明、浸蜡、包埋、切片，用于脱钙的试剂很多，脱钙剂包括有机酸、无机酸、乙二胺四乙酸(EDTA)以及电解法脱钙。

Von Ebener's 液主要由氯化钠、酸等组成，其优点是：①细胞核染色结果好；②无需水洗；其缺点是：①不宜用化学方法确定脱钙终点。该试剂仅用于科研领域，不适用于临床诊断或其他用途。

自备材料：
1、 PBS、蒸馏水
2、 加热装置或微波炉、脱钙专用容器(玻璃)

操作步骤(仅供参考)：
1、骨组织脱钙时，取材不易过厚，一般大约 5mm。
2、组织固定后，用 PBS 清洗 3 次，每次 20min。
3、组织用蒸馏水洗清洗 3 次，每次 20min。
4、组织转移至 20~30 倍体积的 Von Ebener's 液中，脱钙 3～7 天或更长时间。
5、用蒸馏水冲洗数次，常规脱水、包埋。 注意事项：
1、 厚度 5mm 的骨组织块脱钙时间一般脱钙 3~7 天即可。
2、 适当加温能加快脱钙的速度，一般不应超过 37～40℃，温度过高容易使骨组织造成松散解体，尤其不可大于 60℃。
3、 脱钙应彻底，防止脱钙不足或过度；脱钙程度应控制在不影响组织切片的同时尽量缩短脱钙时间，以免脱钙过长引起组织损害。
4、 脱钙用具避免使用金属容器，尽量使用玻璃容器

Product Introduction:


When some tissues contain bone or calcification foci in the tissue sectioning process, the calcium-containing tissues should not be directly embedded with paraffin, because the density between calcium and paraffin is different, and it is difficult to cut a complete section. It is best to fix the calcium-containing tissue, then decalcification or both, and then downstream operations such as dehydration, transparent, wax dipping, embedding, and section. There are many reagents used for decalcification, including organic acid, inorganic acid, EDTA, and electrolysis decalcification.


Von Ebener's solution is mainly composed of sodium chloride, acid, etc. Its advantages are: (1) Good nuclear staining results; No need to wash; The disadvantages are: ① it is not suitable to determine the end point of decalcification by chemical methods. The reagent is only used in the field of scientific research and is not intended for clinical diagnosis or other purposes.


Self-provided materials:
1, PBS, distilled water
2. Heating device or microwave oven, special container for decalcification (glass)


Operation steps (for reference only) :
1, when bone tissue decalcification, the material is not easy to be too thick, generally about 5mm.
2. After the tissue is fixed, clean with PBS for 3 times, 20min each time.
3. Wash the tissue with distilled water for 3 times, 20min each time.
4, tissue transfer to 20 to 30 times the volume of Von Ebener's solution, decalcification for 3 to 7 days or more.
5, washed with distilled water several times, conventional dehydration, embedding. Note:
1. The decalcification time of bone tissue with a thickness of 5mm is generally 3 to 7 days.
2, appropriate heating can speed up the speed of decalcification, generally should not exceed 37 ~ 40℃, the temperature is too high, easy to cause loose disintegration of bone tissue, especially not greater than 60℃.
3, decalcification should be thorough, to prevent insufficient or excessive decalcification; The degree of decalcification should be controlled to minimize the time of decalcification without affecting the tissue sections, so as to avoid tissue damage caused by too long decalcification.
4, decalcification appliances avoid the use of metal containers, try to use glass containers