PH1071 | 精子稀释液（计数液） Sperm Dilution Counting solution

原理

将精液用稀释液作一定量稀释后，精子被杀死，注入计数池在显微镜下计数，然后计算出精液中精子数。该稀释液仅适用于科研领域，不适用于临床诊断或其他用途。

操作（可具体根据自己实验或者相关文献操作）

1、于清洁小试管中加入精子稀释液0.38ml。

2、取经37℃30分钟液化的精液20ul，擦去管外附着的精液，置于精子稀释液内，混匀。

3、取上述混匀的悬液加至血球计数池内,静置3-5分钟。

4、用低倍镜计数左上、左下、右上、右下及中央五个中方格，五个中方格总数乘以10⁹/L即为每升精子数。

正常参考值: 100-150x10⁹/L

常温运输与保存，有效期12个月

Principle

After diluting the semen with a certain amount of diluent, the sperm are killed and injected into the counting chamber for counting under a microscope. Then, the number of sperm in the semen is calculated. This dilution solution is only applicable for scientific research and is not suitable for clinical diagnosis or other

uses.

Operating reference (can be operated specifically according to your own experiment or relevant literature)

1. Add 0.38 ml of sperm diluent to a clean small test tube.

2. Take 20 μl of semen that has been liquefied at 37℃ for 30 minutes, wipe off the semen adhering to the outside of the tube, and place it in the sperm diluent, then mix well.

3. Take the above mixed suspension and add it to the hemocytometer, and let it stand for 3-5 minutes.

4. Count the five middle squares on the left upper, left lower, right upper, right lower and central parts with a low-power microscope. The total number of the five middle squares multiplied by 109/L is the number of sperm per liter.

Normal reference range: 100 - 150 x 109/L

Transport and storage at room temperature. Valid for 12 months.